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Cholinergic receptors in the murine oviduct: inventory and coupling to intracellular calcium concentration

Item Type:Article
Title:Cholinergic receptors in the murine oviduct: inventory and coupling to intracellular calcium concentration
Creators Name:Wolff, M. and Noreikat, K. and Ibanez-Tallon, I. and Lips, K.S. and Koelle, S. and Kummer, W.
Abstract:AIMS: In the oviduct, muscarinic acetylcholine receptors (MR) are linked with motility regulation and nicotinic receptors (nAChR) with ectopic pregnancy. We here aimed to determine the repertoire of cholinergic receptor expression in the murine oviduct and their functional coupling to regulation of intracellular calcium concentration ([Ca(2+)](i)). MAIN METHODS: Cholinergic receptor transcripts were assessed by RT-PCR in oviductal segments (ampulla, isthmus, uterotubar junction) in all cyclic stages and pregnancy, and in laser-microdissected samples of epithelium and smooth muscle, nAChR subunit α3 distribution in tissue sections using an appropriate genetic reporter mouse strain. [Ca(2+)](i) responses were monitored in ciliated and non-ciliated oviductal cells isolated from wild-type and MR subtypes 1 and 3 gene deficient mice. KEY FINDINGS: Transcripts for all MR subtypes (M1-M5) are constantly expressed whereas there is some variability in nAChR expression from individual to individual. The qualitative expression pattern is independent from the hormonal status of the animal, except for nAChR α7, which is less present during pregnancy. The epithelium expresses M1, M3, nAChR α7 (data from laser-assisted microdissection) and nAChR α3 (ultrastructural investigation of reporter mice). MR dominate over nAChR in increasing [Ca(2+)](i) with being M3 the major, but not sole subtype driving this effect. The general nAChR inhibitor mecamylamine enhances muscarinic and purinergic responses. SIGNIFICANCE: In conclusion, the murine oviduct is endowed with a multiplicity of muscarinic and nicotinic receptors subtypes that, with respect to regulation of [Ca(2+)](i), are inversely linked to each other. The major, but not sole, cholinergic receptor driving increase in [Ca(2+)](i) is M3.
Keywords:Oviduct, Non-Neuronal Cholinergic System, Muscarinic Receptors, Nicotinic Receptors, Animals, Mice
Source:Life Sciences
ISSN:0024-3205
Publisher:Elsevier (U.K.)
Volume:91
Number:21-22
Page Range:1003-1008
Date:27 November 2012
Official Publication:https://doi.org/10.1016/j.lfs.2012.03.016
PubMed:View item in PubMed

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