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RBM20, a gene for hereditary cardiomyopathy, regulates titin splicing

Item Type:Article
Title:RBM20, a gene for hereditary cardiomyopathy, regulates titin splicing
Creators Name:Guo, W. and Schafer, S. and Greaser, M.L. and Radke, M.H. and Liss, M. and Govindarajan, T. and Maatz, H. and Schulz, H. and Lincoln, S.E. and Parrish, A.M. and Dauksaite, V. and Vakeel, P. and Klaassen, S. and Gerull, B. and Thierfelder, L. and Regitz-Zagrosek, V. and Hacker, T.A. and Saupe, K.W. and Dec, G.W. and Ellinor, P.T. and MacRae, C.A. and Spallek, B. and Fischer, R. and Perrot, A. and Ozcelik, C. and Saar, K. and Hubner, N. and Gotthardt, M.
Abstract:Alternative splicing has a major role in cardiac adaptive responses, as exemplified by the isoform switch of the sarcomeric protein titin, which adjusts ventricular filling. By positional cloning using a previously characterized rat strain with altered titin mRNA splicing, we identified a loss-of-function mutation in the gene encoding RNA binding motif protein 20 (Rbm20) as the underlying cause of pathological titin isoform expression. The phenotype of Rbm20-deficient rats resembled the pathology seen in individuals with dilated cardiomyopathy caused by RBM20 mutations. Deep sequencing of the human and rat cardiac transcriptome revealed an RBM20-dependent regulation of alternative splicing. In addition to titin (TTN), we identified a set of 30 genes with conserved splicing regulation between humans and rats. This network is enriched for genes that have previously been linked to cardiomyopathy, ion homeostasis and sarcomere biology. Our studies emphasize the key role of post-transcriptional regulation in cardiac function and provide mechanistic insights into the pathogenesis of human heart failure.
Keywords:Base Sequence, Dilated Cardiomyopathy, Inbred BN Rats, Inbred F344 Rats, LIM Domain Proteins, Molecular Sequence Data, Muscle Proteins, Mutation, Protein Kinases, RNA Splicing, RNA-Binding Proteins, Signal Transducing Adaptor Proteins, Animals, Rats
Source:Nature Medicine
Publisher:Nature Publishing Group
Page Range:766-773
Date:May 2012
Official Publication:https://doi.org/10.1038/nm.2693
PubMed:View item in PubMed

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