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Identification of Alzheimer disease risk genotype that predicts efficiency of SORL1 expression in the brain

Item Type:Article
Title:Identification of Alzheimer disease risk genotype that predicts efficiency of SORL1 expression in the brain
Creators Name:Caglayan, S. and Bauerfeind, A. and Schmidt, V. and Carlo, A.S. and Prabakaran, T. and Huebner, N. and Willnow, T.E.
Abstract:OBJECTIVE: To identify SORL1 risk genotypes that determine receptor protein expression in the human brain. DESIGN: DNA, RNA, and proteins were extracted from brain autopsies of Alzheimer disease cases and used for SORL1 genotyping, RNA profiling, and SORLA protein quantification, respectively. SETTING: Specimens were provided by the MRC London Brain Bank for Neurodegenerative Diseases and the Netherlands Brain Bank. Subjects Brain autopsy material (frontal cortex) from 88 confirmed cases of sporadic Alzheimer disease. RESULTS: Our studies identified a SORL1 haplotype in the 3' gene region consisting of single-nucleotide polymorphisms rs1699102 and rs2070045 that is associated with poor receptor expression in the brain of patients with Alzheimer disease. These gene variations alter the SORL1 transcript sequence, resulting in a change from frequent to rare codon usage in the minor risk genotype. Studies in cultured cells confirm less efficient translation of the minor receptor transcripts into protein. CONCLUSION: Our findings suggest a functional mechanism that correlates SORL1 genotype with efficiency of receptor expression in the human brain.
Keywords:Alleles, Alzheimer Disease, Autopsy, Brain, Brain Chemistry, Cultured Cells, DNA, Enzyme-Linked Immunosorbent Assay, Genetic Variation, Genotype, Haplotypes, Humans, LDL-Receptor Related Proteins, Membrane Transport Proteins, Predictive Value of Tests, RNA, Risk Assessment, Single Nucleotide Polymorphism, Western Blotting
Source:Archives of Neurology
ISSN:0003-9942
Publisher:American Medical Association (U.S.A.)
Volume:69
Number:3
Page Range:373-379
Date:March 2012
Official Publication:https://doi.org/10.1001/archneurol.2011.788
PubMed:View item in PubMed

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