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Combined RNAi and localization for functionally dissecting long noncoding RNAs

Item Type:Article
Title:Combined RNAi and localization for functionally dissecting long noncoding RNAs
Creators Name:Chakraborty, D. and Kappei, D. and Theis, M. and Nitzsche, A. and Ding, L. and Paszkowski-Rogacz, M. and Surendranath, V. and Berger, N. and Schulz, H. and Saar, K. and Hubner, N. and Buchholz, F.
Abstract:Whereas methods to comprehensively study cellular roles of protein-coding genes are available, techniques to systematically investigate long noncoding RNAs (lncRNAs), which have been implicated in diverse biological pathways, are limited. Here we report combined knockdown and localization analysis of noncoding RNAs (c-KLAN) that merges functional characterization and localization approaches to study lncRNAs. Using this technique we identified transcripts that regulate mouse embryonic stem cell identity.
Keywords:Embryonic Stem Cells, Fluorescence In Situ Hybridization, Gene Knockdown Techniques, RNA Interference, Untranslated RNA, Animals, Mice
Source:Nature Methods
Publisher:Nature Publishing Group
Page Range:360-362
Date:12 February 2012
Official Publication:https://doi.org/10.1038/nmeth.1894
PubMed:View item in PubMed

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