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The ubiquitin ligase Rnf6 regulates local LIM kinase 1 levels in axonal growth cones

Item Type:Article
Title:The ubiquitin ligase Rnf6 regulates local LIM kinase 1 levels in axonal growth cones
Creators Name:Tursun, B. and Schlueter, A. and Peters, M.A. and Viehweger, B. and Ostendorff, H.P. and Soosairajah, J. and Drung, A. and Bossenz, M. and Johnsen, S.A. and Schweizer, M. and Bernard, O. and Bach, I.
Abstract:LIM kinase 1 (LIMK1) controls important cellular functions such as morphogenesis, cell motility, tumor cell metastasis, development of neuronal projections, and growth cone actin dynamics. We have investigated the role of the RING finger protein Rnf6 during neuronal development and detected high Rnf6 protein levels in developing axonal projections of motor and DRG neurons during mouse embryogenesis as well as cultured hippocampal neurons. RNAi-mediated knock-down experiments in primary hippocampal neurons identified Rnf6 as a regulator of axon outgrowth. Consistent with a role in axonal growth, we found that Rnf6 binds to, polyubiquitinates, and targets LIMK1 for proteasomal degradation in growth cones of primary hippocampal neurons. Rnf6 is functionally linked to LIMK1 during the development of axons, as the changes in axon outgrowth induced by up- or down-regulation of Rnf6 levels can be restored by modulation of LIMK1 expression. Thus, these results assign a specific role for Rnf6 in the control of cellular LIMK1 concentrations and indicate a new function for the ubiquitin/proteasome system in regulating local growth cone actin dynamics.
Keywords:LIM Kinase, Actin Cytoskeleton, Axon Development, Primary Hippocampal Neurons, Protein Stability, Ubiquitin, Proteasome, Animals, Cricetinae, Cricetulus, Cercopithecus Aethiops, Mice
Source:Genes & Development
Publisher:Cold Spring Harbor Laboratory Press
Page Range:2307-2319
Date:1 October 2005
Official Publication:https://doi.org/10.1101/gad.1340605
PubMed:View item in PubMed

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