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Proteasomal selection of multiprotein complexes recruited by LIM homeodomain transcription factors

Item Type:Article
Title:Proteasomal selection of multiprotein complexes recruited by LIM homeodomain transcription factors
Creators Name:Guengoer, C. and Taniguchi-Ishigaki, N. and Ma, H. and Drung, A. and Tursun, B. and Ostendorff, H.P. and Bossenz, M. and Becker, C.G. and Becker, T. and Bach, I.
Abstract:Complexes composed of multiple proteins regulate most cellular functions. However, our knowledge about the molecular mechanisms governing the assembly and dynamics of these complexes in cells remains limited. The in vivo activity of LIM homeodomain (LIM-HD) proteins, a class of transcription factors that regulates neuronal development, depends on the high-affinity association of their LIM domains with cofactor of LIM homeodomain proteins (LIM-HDs) (CLIM, also known as Ldb or NLI). CLIM cofactors recruit single-stranded DNA-binding protein 1 (SSDP1, also known as SSBP3), and this interaction is important for the activation of the LIM-HD/CLIM protein complex in vivo. Here, we identify a cascade of specific protein interactions that protect LIM-HD multiprotein complexes from proteasomal degradation. In this cascade, CLIM stabilizes LIM-HDs, and SSDP1 stabilizes CLIM. Furthermore, we show that stabilizing cofactors prevent binding of ubiquitin ligases to multiple protein interaction domains in LIM-HD recruited protein complexes. Together, our results indicate a combinatorial code that selects specific multiprotein complexes via proteasomal degradation in cells with broad implications for the assembly and specificity of multiprotein complexes.
Keywords:LIM Domain, Proteasome, Protein Complex, Protein Interaction, Ubiquitin
Source:Proceedings of the National Academy of Sciences of the United States of America
Publisher:National Academy of Sciences
Page Range:15000-15005
Date:18 September 2007
Official Publication:https://doi.org/10.1073/pnas.0703738104
PubMed:View item in PubMed

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