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| Item Type: | Article |
|---|---|
| Title: | The Groucho ortholog UNC-37 interacts with the short Groucho-like protein LSY-22 to control developmental decisions in C. elegans |
| Creators Name: | Flowers, E.B. and Poole, R.J. and Tursun, B. and Bashllari, E. and Pe'er, I. and Hobert, O. |
| Abstract: | Transcriptional co-repressors of the Groucho/TLE family are important regulators of development in many species. A subset of Groucho/TLE family members that lack the C-terminal WD40 domains have been proposed to act as dominant-negative regulators of Groucho/TLE proteins, yet such a role has not been conclusively proven. Through a mutant screen for genes controlling a left/right asymmetric cell fate decision in the nervous system of the nematode C. elegans, we have retrieved loss-of-function alleles in two distinct loci that display identical phenotypes in neuronal fate specification and in other developmental contexts. Using the novel technology of whole-genome sequencing, we find that these loci encode the C. elegans ortholog of Groucho, UNC-37, and, surprisingly, a short Groucho-like protein, LSY-22, that is similar to truncated Groucho proteins in other species. Besides their phenotypic similarities, unc-37 and lsy-22 show genetic interactions and UNC-37 and LSY-22 proteins also physically bind to each other in vivo. Our findings suggest that rather than acting as negative regulators of Groucho, small Groucho-like proteins may promote Groucho function. We propose that Groucho-mediated gene regulatory events involve heteromeric complexes of distinct Groucho-like proteins. |
| Keywords: | C. elegans, Left/Right Asymmetry, Neuronal Development, Transcriptional Control, Animals, Caenorhabditis elegans |
| Source: | Development |
| ISSN: | 0950-1991 |
| Publisher: | Company of Biologists |
| Volume: | 137 |
| Number: | 11 |
| Page Range: | 1799-1805 |
| Date: | June 2010 |
| Official Publication: | https://doi.org/10.1242/dev.046219 |
| PubMed: | View item in PubMed |
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