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Loss of R-spondin1 and Foxl2 amplifies female-to-male sex reversal in XX mice

Item Type:Article
Title:Loss of R-spondin1 and Foxl2 amplifies female-to-male sex reversal in XX mice
Creators Name:Auguste, A. and Chassot, A.A. and Gregoire, E.P. and Renault, L. and Pannetier, M. and Treier, M. and Pailhoux, E. and Chaboissier, M.C.
Abstract:In vertebrates, 2 main genetic pathways have been shown to regulate ovarian development. Indeed, a loss of function mutations in Rspo1 and Foxl2 promote partial female-to-male sex reversal. In mice, it has been shown that the secreted protein RSPO1 is involved in ovarian differentiation and the transcription factor FOXL2 is required for follicular formation. Here, we analysed the potential interactions between these 2 genetic pathways and have shown that while Rspo1 expression seems to be independent of Foxl2 up-regulation, Foxl2 expression partly depends of Rspo1 signalisation. This suggests that different Foxl2-positive somatic cell lineages exist within the ovaries. In addition, a combination of both mutated genes in XX Foxl2(-/-)/Rspo1(-/-) gonads promotes sex reversal, detectable at earlier stages than in XX Rspo1(-/-) mutants. Ectopic development of the steroidogenic lineage is more pronounced in XX Foxl2(-/-)/Rspo1(-/-) gonads than in XX Rspo1(-/-) embryos, suggesting that Foxl2 is involved in preventing ectopic steroidogenesis in foetal ovaries.
Keywords:Foxl2, Mouse, Ovarian Differentiation, R-Spondin1, XX Sex-Reversal, Animals, Mice
Source:Sexual Development
Page Range:304-317
Date:22 November 2011
Official Publication:https://doi.org/10.1159/000334517
PubMed:View item in PubMed

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