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Genotype-independent transmission of transgenic fluorophore protein by boar spermatozoa

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Item Type:Article
Title:Genotype-independent transmission of transgenic fluorophore protein by boar spermatozoa
Creators Name:Garrels, W. and Holler, S. and Taylor, U. and Herrmann, D. and Struckmann, C. and Klein, S. and Barg-Kues, B. and Nowak-Imialek, M. and Ehling, C. and Rath, D. and Ivics, Z. and Niemann, H. and Kues, W.A.
Abstract:Recently, we generated transposon-transgenic boars (Sus scrofa), which carry three monomeric copies of a fluorophore marker gene. Amazingly, a ubiquitous fluorophore expression in somatic, as well as in germ cells was found. Here, we characterized the prominent fluorophore load in mature spermatozoa of these animals. Sperm samples were analyzed for general fertility parameters, sorted according to X and Y chromosome-bearing sperm fractions, assessed for potential detrimental effects of the reporter, and used for inseminations into estrous sows. Independent of their genotype, all spermatozoa were uniformly fluorescent with a subcellular compartmentalization of the fluorophore protein in postacrosomal sheath, mid piece and tail. Transmission of the fluorophore protein to fertilized oocytes was shown by confocal microscopic analysis of zygotes. The monomeric copies of the transgene segregated during meiosis, rendering a certain fraction of the spermatozoa non-transgenic (about 10% based on analysis of 74 F1 offspring). The genotype-independent transmission of the fluorophore protein by spermatozoa to oocytes represents a non-genetic contribution to the mammalian embryo.
Keywords:Animals
Source:PLoS ONE
ISSN:1932-6203
Publisher:Public Library of Science (U.S.A.)
Volume:6
Number:11
Page Range:e27563
Date:16 November 2011
Official Publication:https://doi.org/10.1371/journal.pone.0027563
PubMed:View item in PubMed

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