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Cre-mediated somatic site-specific recombination in mice

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Item Type:Article
Title:Cre-mediated somatic site-specific recombination in mice
Creators Name:Akagi, K. and Sandig, V. and Vooijs, M. and van der Valk, M. and Giovannini, M. and Strauss, M. and Berns, A.
Abstract:Conditional mutant mice equipped with heterologous recombination systems (Cre/lox or Flp/frt) are promising for studying tissue-specific gene function and for designing better models of human diseases. The utility of these mice depends on the cell target specificity, on the efficiency and on the control over timing of gene (in)activation. We have explored the utility of adenoviral vectors and transgenic mice expressing Cre under the control of tissue-specific promoters to achieve Cre/lox-mediated somatic recombination of the LacZ reporter gene, using a newly generated flox LacZ mouse strain. When adeno Cre viruses were administered via different routes, recombination and expression of LacZ was detected in a wide range of tissues. Whereas in liverbeta-galactosidase activity was quickly lost by turnover of expressing cells, even though the recombined allele was retained,beta-galactosidase in other tissues persisted for many months. Our data indicate that the flox LacZ transgenic line can be utilized effectively to monitor the level and functionality of Cre protein produced upon infection with adeno Cre virus or upon crossbreeding with different Cre transgenic lines.
Keywords:Actins, Adenoviridae, Chloramphenicol O-Acetyltransferase, Reporter Genes, Integrases, Lac Operon, Transgenic MIce, Genetic Recombination, Viral Proteins, beta-Galactosidase, Animals, Mice
Source:Nucleic Acids Research
Publisher:Oxford University Press
Page Range:1766-1773
Date:1 May 1997
Official Publication:http://nar.oxfordjournals.org/cgi/content/abstract/25/9/1766
PubMed:View item in PubMed

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