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GTP-dependent scaffold formation in the GTPase of Immunity Associated Protein family

Item Type:Article
Title:GTP-dependent scaffold formation in the GTPase of Immunity Associated Protein family
Creators Name:Schwefel, D. and Daumke, O.
Abstract:GTP ases of Immunity-Associated Proteins (GIMAPs) are a family of guanine nucleotide binding (G) proteins which are implicated in the regulation of apoptosis in lymphocytes. GIMAPs are composed of an amino-terminal G domain and carboxy-terminal extensions of varying size. Our recent biochemical and structural analysis of a representative GIMAP family member, GIMAP2, revealed the molecular basis of GTP-dependent oligomerization which involves two interfaces in the G domain. Whereas the amphipathic helix α7 in the C-terminal extension closely folds against the G domain in the GDP-bound state, it might be released in the GTP-bound state to assemble interaction partners. We also showed that the GIMAP2 oligomer functions at the surface of lipid droplets in a Jurkat T cell line. Here, we review our recent work and discuss the GIMAP2 oligomer as a GTP-dependent protein scaffold at the surface of lipid droplets controlling apoptosis.
Keywords:GIMAP, GTPase, Protein Scaffold, Lipid Droplets, Lymphocytes, Protein Structure, Immunity, Animals, Mice
Source:Small GTPases
ISSN:2154-1248
Publisher:Landes Bioscience
Volume:2
Number:1
Page Range:27-30
Date:January 2011
Official Publication:https://doi.org/10.4161/sgtp.2.1.14938
PubMed:View item in PubMed

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