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MEK1 binds directly to betaarrestin1, influencing both its phosphorylation by ERK and the timing of its isoprenaline-stimulated internalization

Item Type:Article
Title:MEK1 binds directly to betaarrestin1, influencing both its phosphorylation by ERK and the timing of its isoprenaline-stimulated internalization
Creators Name:Meng, D. and Lynch, M.J. and Huston, E. and Beyermann, M. and Eichhorst, J. and Adams, D.R. and Klussmann, E. and Houslay, M.D. and Baillie, G.S.
Abstract:betaArrestin is a multifunctional signal scaffold protein. Using SPOT immobilized peptide arrays, coupled with scanning alanine substitution and mutagenesis, we show that the MAPK kinase, MEK1, interacts directly with betaarrestin1. Asp(26) and Asp(29) in the N-terminal domain of betaarrestin1 are critical for its binding to MEK1, whereas Arg(47) and Arg(49) in the N-terminal domain of MEK1 are critical for its binding to betaarrestin1. Wild-type FLAG-tagged betaarrestin1 co-immunopurifies with MEK1 in HEKB2 cells, whereas the D26A/D29A mutant does not. ERK-dependent phosphorylation at Ser(412) was compromised in the D26A/D29A-betaarrestin1 mutant. A cell-permeable, 25-mer N-stearoylated betaarrestin1 peptide that encompassed the N-domain MEK1 binding site blocked betaarrestin1/MEK1 association in HEK cells and recapitulated the altered phenotype seen with the D26A/D29A-betaarrestin1 in compromising the ERK-dependent phosphorylation of betaarrestin1. In addition, the MEK disruptor peptide promoted the ability of betaarrestin1 to co-immunoprecipitate with endogenous c-Src and clathrin, facilitating the isoprenaline-stimulated internalization of the beta(2)-adrenergic receptor.
Keywords:Adrenergic beta-Agonists, Amino Acid Sequence, Arginine, Arrestins, Aspartic Acid, Clathrin, Isoproterenol, MAP Kinase Kinase 1, Biological Models, Molecular Sequence Data, Phosphorylation, Tertiary Protein Structure, Amino Acid Sequence Homology, src-Family Kinases
Source:Journal of Biological Chemistry
ISSN:0021-9258
Publisher:American Society for Biochemistry and Molecular Biology (U.S.A.)
Volume:284
Number:17
Page Range:11425-1135
Date:24 April 2009
Additional Information:Erratum in: JBC 284 (2009): 11425–11435
Official Publication:https://doi.org/10.1074/jbc.M806395200
PubMed:View item in PubMed

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