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Angioprotectin: an angiotensin II-like peptide causing vasodilatory effects

Item Type:Article
Title:Angioprotectin: an angiotensin II-like peptide causing vasodilatory effects
Creators Name:Jankowski, V. and Toelle, M. and Santos, R.A. and Guenthner, T. and Krause, E. and Beyermann, M. and Welker, P. and Bader, M. and Pinheiro, S.V.B. and Sampaio, W.O. and Lautner, R. and Kretschmer, A. and van der Giet, M. and Zidek, W. and Jankowski, J.
Abstract:The family of angiotensin peptides has been steadily growing in recent years. Most are fragments of angiotensin II (Ang II) with different affinities to the known angiotensin receptors. Here, we describe a novel endogenous Ang II-like octapeptide in plasma from healthy humans and patients with end-stage renal failure, which acts as a stronger agonist at Mas receptors than Ang 1-7. Chromatographic purification and structural analysis by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight (MALDI-TOF/TOF) revealed an Ang II-like octapeptide, angioprotectin, with the sequence Pro-Glu-Val-Tyr-Ile-His-Pro-Phe, which differs from Ang II in Pro(1) and Glu(2) instead of Asp(1) and Arg(2). Pro-Glu-Val-Tyr-Ile-His-Pro-Phe in angioprotectin is most likely generated enzymatically from Ang II. Angioprotectin antagonized the contractile actions of Ang II on rat aortic rings. The physiological antagonism of vasoconstrictor actions of Ang II by angioprotectin is mediated by the Mas receptor. Angioprotectin has a stronger affinity to the Mas receptor than Ang-1-7. Plasma concentrations were ~15% of plasma Ang II concentrations in healthy volunteers and up to 50% in patients with renal failure. A commercially available Ang II antibody did not discriminate between angioprotectin and Ang II; thus, angioprotectin can contribute to Ang II concentrations measured by antibody-based assays. This novel peptide is likely to be a relevant component of the human renin-angiotensin-system.
Keywords:Amino Acid Exchange, Vascular Tone, Animals, Mice, Rats
Source:FASEB Journal
Publisher:Federation of American Societies for Experimental Biology
Page Range:2987-2995
Date:September 2011
Official Publication:https://doi.org/10.1096/fj.11-185470
PubMed:View item in PubMed

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