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E-cadherin is crucial for embryonic stem cell pluripotency and can replace OCT4 during somatic cell reprogramming

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Item Type:Article
Title:E-cadherin is crucial for embryonic stem cell pluripotency and can replace OCT4 during somatic cell reprogramming
Creators Name:Redmer, T. and Diecke, S. and Grigoryan, T. and Quiroga-Negreira, A. and Birchmeier, W. and Besser, D.
Abstract:We report new functions of the cell-adhesion molecule E-cadherin in murine pluripotent cells. E-cadherin is highly expressed in mouse embryonic stem cells, and interference with E-cadherin causes differentiation. During cellular reprogramming of mouse fibroblasts by OCT4, SOX2, KLF4 and c-MYC, fully reprogrammed cells were exclusively observed in the E-cadherin-positive cell population and could not be obtained in the absence of E-cadherin. Moreover, reprogrammed cells could be established by viral E-cadherin in the absence of exogenous OCT4. Thus, reprogramming requires spatial cues that cross-talk with essential transcription factors. The cell-adhesion molecule E-cadherin has important functions in pluripotency and reprogramming.
Keywords:Pluripotency, Somatic Cell Reprogramming, E-Cadherin, OCT4, Cadherins, Cell Differentiation, Cell Line, Developmental Gene Expression Regulation, Embryonic Stem Cells, Gene Deletion, Messenger RNA, Nuclear Reprogramming, Octamer Transcription Factor-3, Pluripotent Stem Cells, Animals, Mice, 129 Strain Mice, Inbred NOD Mice, SCID Mice
Source:EMBO Reports
ISSN:1469-221X
Publisher:Nature Publishing Group (U.K.)
Volume:12
Number:7
Page Range:720-726
Date:1 July 2011
Official Publication:https://doi.org/10.1038/embor.2011.88
PubMed:View item in PubMed

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