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Homo- and heterodimerization of APP family members promotes intercellular adhesion

Item Type:Article
Title:Homo- and heterodimerization of APP family members promotes intercellular adhesion
Creators Name:Soba, P. and Eggert, S. and Wagner, K. and Zentgraf, H. and Siehl, K. and Kreger, S. and Loewer, A. and Langer, A. and Merdes, G. and Paro, R. and Masters, C.L. and Mueller, U. and Kins, S. and Beyreuther, K.
Abstract:The amyloid precursor protein (APP) plays a central role in Alzheimer's disease, but its physiological function and that of its mammalian paralogs, the amyloid precursor-like proteins 1 and 2 (APLPs), is still poorly understood. APP has been proposed to form dimers, a process that could promote cell adhesion via trans-dimerization. We investigated the dimerization and cell adhesion properties of APP/APLPs and provide evidence that all three paralogs are capable of forming homo- and heterocomplexes. Moreover, we show that trans-interaction of APP family proteins promotes cell-cell adhesion in a homo- and heterotypic fashion and that endogenous APLP2 is required for cell-cell adhesion in mouse embryonic fibroblasts. We further demonstrate interaction of all the three APP family members in mouse brain, genetic interdependence, and molecular interaction of APP and APLPs in synaptically enriched membrane compartments. Together, our results provide evidence that homo- and heterocomplexes of APP/APLPs promote trans-cellular adhesion in vivo.
Keywords:Alzheimer's Disease, APP, Cell Adhesion, Dimerization, Animals, Mice
Source:EMBO Journal
Publisher:Nature Publishing Group
Page Range:3624-3634
Date:19 October 2005
Additional Information:Erratum in: EMBO J. 2006 Feb 8;25(3):653.
Official Publication:https://doi.org/10.1038/sj.emboj.7600824
PubMed:View item in PubMed

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