Helmholtz Gemeinschaft


Kainate promotes alterations in neuronal RNA splicing machinery

Item Type:Article
Title:Kainate promotes alterations in neuronal RNA splicing machinery
Creators Name:Rohe, M. and Nebrich, G. and Klein, O. and Mao, L. and Zabel, C. and Klose, J. and Hartl, D.
Abstract:Kainate, a glutamate analogue, activates kainate and AMPA receptors inducing strong synaptic activation. Systemic kainate application to rodents results in seizures, neurodegeneration, and neuronal remodeling in the brain. It is therefore used to investigate molecular mechanisms responsible for these conditions. We analyzed proteome alterations in murine primary cortical neurons after 24 h of kainate treatment. Our 2-D gel based proteomics approach revealed 91 protein alterations, some already associated with kainate-induced pathology. In addition, we found a large number of proteins which have not previously been reported to be associated with kainate-induced pathology. Functional classification of altered proteins revealed that they predominantly participate in mRNA splicing and cytoskeleton remodeling.
Keywords:Kainic Acid, Kainate, Epilepsy, Primary Neuron, Proteome, BDNF, Glutamate, Alternative Splicing, Neurodegeneration, Animals, Mice, Rats
Source:Journal of Proteome Research
Publisher:American Chemical Society
Page Range:1459-1467
Date:1 April 2011
Official Publication:https://doi.org/10.1021/pr101008p
PubMed:View item in PubMed

Repository Staff Only: item control page

Open Access
MDC Library