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Adenine nucleotide translocase-1, a component of the permeability transition pore, can dominantly induce apoptosis

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Item Type:Article
Title:Adenine nucleotide translocase-1, a component of the permeability transition pore, can dominantly induce apoptosis
Creators Name:Bauer, M.K.A. and Schubert, A. and Rocks, O. and Grimm, S.
Abstract:Here, we describe the isolation of adenine nucleotide translocase-1 (ANT-1) in a screen for dominant, apoptosis-inducing genes. ANT-1 is a component of the mitochondrial permeability transition complex, a protein aggregate connecting the inner with the outer mitochondrial membrane that has recently been implicated in apoptosis. ANT-1 expression led to all features of apoptosis, such as phenotypic alterations, collapse of the mitochondrial membrane potential, cytochrome c release, caspase activation, and DNA degradation. Both point mutations that impair ANT-1 in its known activity to transport ADP and ATP as well as the NH(2)-terminal half of the protein could still induce apoptosis. Interestingly, ANT-2, a highly homologous protein could not lead to cell death, demonstrating the specificity of the signal for apoptosis induction. In contrast to Bax, a proapoptotic Bcl-2 gene, ANT-1 was unable to elicit a form of cell death in yeast. This and the observed repression of apoptosis by the ANT-1-interacting protein cyclophilin D suggest that the suicidal effect of ANT-1 is mediated by specific protein-protein interactions within the permeability transition pore.
Keywords:Apoptosis, Cell Death, Cell Division, Cell Line, Cyclophilins, Immunophilins, Intracellular Membranes, Membrane Potentials, Mitochondria, ATP Translocases Mitochondrial ADP, Peptide Fragments, Permeability, Saccharomyces cerevisiae, Animals, Cricetinae, Mice
Source:Journal of Cell Biology
ISSN:0021-9525
Publisher:Rockefeller University Press (U.S.A.)
Volume:147
Number:7
Page Range:1493-1502
Date:27 December 1999
Official Publication:https://doi.org/10.1083/jcb.147.7.1493
PubMed:View item in PubMed

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