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An acylation cycle regulates localization and activity of palmitoylated Ras isoforms

Item Type:Article
Title:An acylation cycle regulates localization and activity of palmitoylated Ras isoforms
Creators Name:Rocks, O. and Peyker, A. and Kahms, M. and Verveer, P.J. and Koerner, C. and Lumbierres, M. and Kuhlmann, J. and Waldmann, H. and Wittinghofer, A. and Bastiaens, P.I.
Abstract:We show that the specific subcellular distribution of H- and Nras guanosine triphosphate-binding proteins is generated by a constitutive de/reacylation cycle that operates on palmitoylated proteins, driving their rapid exchange between the plasma membrane (PM) and the Golgi apparatus. Depalmitoylation redistributes farnesylated Ras in all membranes, followed by repalmitoylation and trapping of Ras at the Golgi, from where it is redirected to the PM via the secretory pathway. This continuous cycle prevents Ras from nonspecific residence on endomembranes, thereby maintaining the specific intracellular compartmentalization. The de/reacylation cycle also initiates Ras activation at the Golgi by transport of PM-localized Ras guanosine triphosphate. Different de/repalmitoylation kinetics account for isoform-specific activation responses to growth factors.
Keywords:Acylation, Amino Acid Sequence, COS Cells, Cell Line, Cell Membrane, Golgi Apparatus, Guanosine Triphosphate, Kinetics, Biological Models, Molecular Sequence Data, Palmitic Acid, Protein Isoforms, Post-Translational Protein Processing, Tertiary Protein Structure, Protein Transport, Proto-Oncogene Proteins p21(ras), Recombinant Fusion Proteins, Transfection, Animals, Cercopithecus Aethiops, Dogs
Publisher:American Association for the Advancement of Science
Page Range:1746-1752
Date:18 March 2005
Official Publication:https://doi.org/10.1126/science.1105654
PubMed:View item in PubMed

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