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Insertional engineering of chromosomes with Sleeping Beauty transposition: an overview

Item Type:Article
Title:Insertional engineering of chromosomes with Sleeping Beauty transposition: an overview
Creators Name:Grabundzija, I. and Izsvak, Z. and Ivics, Z.
Abstract:Novel genetic tools and mutagenesis strategies based on the Sleeping Beauty (SB) transposable element are currently under development with a vision to link primary DNA sequence information to gene functions in vertebrate models. By virtue of its inherent capacity to insert into DNA, the SB transposon can be developed into powerful tools for chromosomal manipulations. Mutagenesis screens based on SB have numerous advantages including high throughput and easy identification of mutated alleles. Forward genetic approaches based on insertional mutagenesis by engineered SB transposons have the advantage of providing insight into genetic networks and pathways based on phenotype. Indeed, the SB transposon has become a highly instrumental tool to induce tumors in experimental animals in a tissue-specific -manner with the aim of uncovering the genetic basis of diverse cancers. Here, we describe a battery of mutagenic cassettes that can be applied in conjunction with SB transposon vectors to mutagenize genes, and highlight versatile experimental strategies for the generation of engineered chromosomes for loss-of-function as well as gain-of-function mutagenesis for functional gene annotation in vertebrate models.
Keywords:Transposon, Transgenesis, Insertional Mutagenesis, Gene Trap, Cre/loxP, Cancer, Embryonic Stem Cells, Animals
Source:Methods in Molecular Biology
Publisher:Springer / Humana Press
Page Range:69-85
Official Publication:https://doi.org/10.1007/978-1-61779-099-7_5
PubMed:View item in PubMed

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