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Molecular imaging of breast cancer in a transgene mouse model [Molekulare Bildgebung des Mammakarzinoms in einem transgenen Mausmodell]

Item Type:Book Section
Title:Molecular imaging of breast cancer in a transgene mouse model [Molekulare Bildgebung des Mammakarzinoms in einem transgenen Mausmodell]
Creators Name:Jakob, J. and Perlitz, C. and Ebert, B. and Klamm, U. and Kemmner, W. and Haensch, W. and Macdonald, R. and Licha, K. and Schirner, M. and Schlag, P.M. and Moesta, K.T.
Abstract:Background: Purpose of the study was to detect early breast cancer and its precursors by in vivo optical molecular imaging in an animal model. Material and methods: Females of the transgene mouse strain WAP-TNP8 develop non-invasive and consequently invasive tumours of the mammary glands due to specific expression of the viral SV40 large tumour antigen induced by lactation. The molecular target for imaging was extradomain-b fibronectin (EDB-FN), which is associated with tumour angiogenesis. The optical probe was designed as a compound of an anti-EDB-FN antibody fragment and a near-infrared fluorescent dye. 30h after intravenous injection of the contrast agent, optical imaging was performed using a pulsed Laser system for excitation and an intensified CCD-camera to record fluorescence images. After optical molecular imaging all animals were sacrified and the tumours were examined histologically. Results: Initiated transgene female animals developed palpable masses of the mammary gland within 6 months (median 4 months). Imaging was performed in 5 animals with a total of 9 tumours (diameter 2–7mm, median 4mm). Applying optical molecular imaging 8 of 9 tumours were detected. The urogenital tract was contrasted unspecifically. Histological examination proved invasive epithelial tumours of the mammary gland in all cases. Conclusion: Breast cancer can be detected in vivo by near-infrared fluorescence molecular imaging targeting neoangiogenesis in a transgene mouse-model.
Source:Chirurgisches Forum 2005
Series Name:Deutsche Gesellschaft fuer Chirurgie
Title of Book:Chirurgisches Forum 2005 : fuer experimentelle und klinische Forschung 122. Kongress der Deutschen Gesellschaft fuer Chirurgie Berlin, 05.04.-08.04.2005
Page Range:83-85
Official Publication:https://doi.org/10.1007/b137791

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