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Identification of early molecular markers for breast cancer

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Item Type:Article
Title:Identification of early molecular markers for breast cancer
Creators Name:Kretschmer, C. and Sterner-Kock, A. and Siedentopf, F. and Schoenegg, W. and Schlag, P.M. and Kemmner, W.
Abstract:BACKGROUND: The ductal carcinoma in situ (DCIS) of the mammary gland represents an early, pre-invasive stage in the development of invasive breast carcinoma. Since DCIS is a curable disease, it would be highly desirable to identify molecular markers that allow early detection. Mice transgenic for the WAP-SV40 early genome region were used as a model for DCIS development. Gene expression profiling was carried out on DCIS-bearing mice and control animals. Additionally, a set of human DCIS and invasive mammary tumors were analyzed in a similar fashion. Enhanced expression of these marker genes in human and murine samples was validated by quantitative RT-PCR. Besides, marker gene expression was also validated by immunohistochemistry of human samples. Furthermore in silico analyses using an online microarray database were performed. RESULTS: In DCIS-mice seven genes were identified that were significantly up-regulated in DCIS: DEPDC1, NUSAP1, EXO1, RRM2, FOXM1, MUC1 and SPP1. A similar up-regulation of homologues of the murine genes was observed in human DCIS samples. Enhanced expression of these genes in DCIS and IDC (invasive ductal carcinoma) was validated by quantitative RT-PCR and immunohistochemistry. Furthermore, the genes were associated with a poor prognosis. CONCLUSIONS: By comparing murine markers for the ductal carcinoma in situ (DCIS) of the mammary gland with genes up-regulated in human DCIS-samples we were able to identify a set of genes which might allow early detection of DCIS and invasive carcinomas. The similarities between gene expression in DCIS and invasive carcinomas suggest that the early detection and treatment of DCIS is of utmost relevance for the survival of patients who are at high risk of developing breast carcinomas.
Keywords:Breast Neoplasms, Breast Ductal Carcinoma, Case-Control Studies, Experimental Mammary Neoplasms, Microarray Analysis, Neoplasm Invasiveness, Reverse Transcriptase Polymerase Chain Reaction, Biological Tumor Markers, Animals, Mice
Source:Molecular Cancer
ISSN:1476-4598
Publisher:BioMed Central (U.K.)
Volume:10
Number:1
Page Range:15
Date:11 February 2011
Official Publication:https://doi.org/10.1186/1476-4598-10-15
PubMed:View item in PubMed

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