Epicatechin gallate and catechin gallate are superior to epigallocatechin gallate in growth suppression and anti-inflammatory activities in pancreatic tumor cells

Item Type:	Article
Title:	Epicatechin gallate and catechin gallate are superior to epigallocatechin gallate in growth suppression and anti-inflammatory activities in pancreatic tumor cells
Creators Name:	Kuerbitz, C. and Heise, D. and Redmer, T. and Goumas, F. and Arlt, A. and Lemke, J. and Rimbach, G. and Kalthoff, H. and Trauzold, A.
Abstract:	Green tea catechins are considered as possible cancer preventive agents for several cancer types but little is known regarding their effects on pancreatic cancer cells. The best studied catechin and the major polyphenol present in green tea is epigallocatechin gallate (EGCG). In the present study, we investigated the in vitro anti-tumoral properties of EGCG on human PDAC (pancreatic ductal adenocarcinoma) cells PancTu-I, Panc1, Panc89 and BxPC3 in comparison with the effects of two minor components of green tea catechins catechin gallate (CG) and epicatechin gallate (ECG). We found that all three catechins inhibited proliferation of PDAC cells in a dose- and time-dependent manner. Interestingly, CG and ECG exerted much stronger anti-proliferative effects than EGCG. Western blot analyses performed with PancTu-I cells revealed catechin-mediated modulation of cell cycle regulatory proteins (cyclins, cyclin-dependent kinases [CDK], CDK inhibitors). Again, these effects were clearly more pronounced in CG or ECG than in EGCG treated cells. Importantly, catechins, in particular ECG, inhibited TNFα-induced activation of NF-κB and consequently secretion of pro-inflammatory and invasion promoting proteins like IL-8 and uPA. Overall, our data show that green tea catechins ECG and CG exhibit potent and much stronger anti-proliferative and anti-inflammatory activities on PDAC cells than the most studied catechin EGCG.
Keywords:	Anti-Inflammatory Agents, Antineoplastic Agents, Catechin, Cell Cycle, Cell Proliferation, Cultured Tumor Cells, Electrophoretic Mobility Shift Assay, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Fluorescent Antibody Technique, Pancreatic Ductal Carcinoma, Pancreatic Neoplasms, Tea, Western Blotting
Source:	Cancer Science
ISSN:	1347-9032
Publisher:	Wiley-Blackwell
Volume:	102
Number:	4
Page Range:	728-734
Date:	April 2011
Official Publication:	https://doi.org/10.1111/j.1349-7006.2011.01870.x
PubMed:	View item in PubMed

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