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Astrocytes control GABAergic inhibition of neurons in the mouse barrel cortex

Item Type:Article
Title:Astrocytes control GABAergic inhibition of neurons in the mouse barrel cortex
Creators Name:Benedetti, B. and Matyash, V. and Kettenmann, H.
Abstract:Astrocytes in the barrel cortex respond with a transient Ca2+ increase to neuronal stimulation and this response is restricted to the stimulated barrel field. In the present study we suppressed the astrocyte response by dialyzing these cells with the Ca2+ chelator BAPTA. Electrical stimulation triggered a depolarization in stellate or pyramidal "regular spiking" neurons from cortex layer 4 and 2/3 and this response was augmented in amplitude and duration after astrocytes were dialyzed with BAPTA. Combined blockade of GABAA and GABAB receptors mimicked the effect of BAPTA dialysis, while glutamate receptor blockers had no effect. Moreover, the frequency of spontaneous postsynaptic currents was increased after BAPTA dialysis. Outside the range of BAPTA dialysis astrocytes responded with a Ca2+ increase, but in contrast to control, the response was no longer restricted to one barrel field. Our findings indicate that astrocytes control neuronal inhibition in the barrel cortex.
Keywords:Astrocyte, Barrel Cortex, Calcium (Ca2+), Animals, Mice
Source:Journal of Physiology
ISSN:0022-3751
Publisher:Wiley-Blackwell (U.K.)
Volume:589
Number:Pt 5
Page Range:1159-1172
Date:1 March 2011
Official Publication:https://doi.org/10.1113/jphysiol.2010.203224
PubMed:View item in PubMed

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