A novel series of anti-human glycophorin A (CD235a) antibodies defining five extra- and intracellular epitopes

Item Type:	Article
Title:	A novel series of anti-human glycophorin A (CD235a) antibodies defining five extra- and intracellular epitopes
Creators Name:	Karsten, U. and Butschak, G. and Stahn, R. and Goletz, S.
Abstract:	Glycophorin A (GPA, CD235a) is a major membrane glycoprotein and marker of cells of the erythroid lineage. It is also the target of Plasmodium falciparum and of influenza virus. We describe a novel series of 10 antibodies towards GPA, recognizing four extra- and intracellular peptide epitopes of this molecule (defined by epitope mapping) and one mixed peptide/carbohydrate epitope. All antibodies bind better to the desialylated than to the fully sialylated molecule, including those specific for the intracellular epitope. For some of the antibodies (representing all five epitopes) functional binding constants were determined by Surface Plasmon Resonance. The new panel complements the already known anti-glycophorin antibodies and offers several potential applications for, e.g., differential diagnosis of erythroleukemias, lineage analyses of erythroid cells, isolation of senescent erythrocytes, or a highly sensitive neuraminidase assay.
Keywords:	Antibodies, Human Glycophorin A, CD235a, Glycosylation, Epitope, Affinity, SPR, Animals, Mice
Source:	International Immunopharmacology
ISSN:	1567-5769
Publisher:	Elsevier
Volume:	10
Number:	11
Page Range:	1354-1360
Date:	November 2010
Official Publication:	https://doi.org/10.1016/j.intimp.2010.08.001
PubMed:	View item in PubMed

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