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| Item Type: | Article |
|---|---|
| Title: | Pseudogenes as an alternative source of natural antisense transcripts |
| Creators Name: | Muro, E.M. and Andrade-Navarro, M.A. |
| Abstract: | BACKGROUND: Naturally occurring antisense transcripts (NATs) are non-coding RNAs that may regulate the activity of sense transcripts to which they bind because of complementarity. NATs that are not located in the gene they regulate (trans-NATs) have better chances to evolve than cis-NATs, which is evident when the sense strand of the cis-NAT is part of a protein coding gene. However, the generation of a trans-NAT requires the formation of a relatively large region of complementarity to the gene it regulates. RESULTS: Pseudogene formation may be one evolutionary mechanism that generates trans-NATs to the parental gene. For example, this could occur if the parental gene is regulated by a cis-NAT that is copied as a trans-NAT in the pseudogene. To support this we identified human pseudogenes with a trans-NAT to the parental gene in their antisense strand by analysis of the database of expressed sequence tags (ESTs). We found that the mutations that appeared in these trans-NATs after the pseudogene formation do not show the flat distribution that would be expected in a non functional transcript. Instead, we found higher similarity to the parental gene in a region nearby the 3' end of the trans-NATs. CONCLUSIONS: Our results do not imply a functional relation of the trans-NAT arising from pseudogenes over their respective parental genes but add evidence for it and stress the importance of duplication mechanisms of genetic material in the generation of non-coding RNAs. We also provide a plausible explanation for the large transcripts that can be found in the antisense strand of some pseudogenes. |
| Keywords: | Antisense RNA, Expressed Sequence Tags, Genetic Databases, Molecular Evolution, Pseudogenes, Animals |
| Source: | BMC Evolutionary Biology |
| ISSN: | 1471-2148 |
| Publisher: | BioMed Central |
| Volume: | 10 |
| Number: | 1 |
| Page Range: | 338 |
| Date: | 3 November 2010 |
| Official Publication: | https://doi.org/10.1186/1471-2148-10-338 |
| PubMed: | View item in PubMed |
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