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AHNAK1 and AHNAK2 are costameric proteins: AHNAK1 affects transverse skeletal muscle fiber stiffness

Item Type:Article
Title:AHNAK1 and AHNAK2 are costameric proteins: AHNAK1 affects transverse skeletal muscle fiber stiffness
Creators Name:Marg, A. and Haase, H. and Neumann, T. and Kouno, M. and Morano, I.
Abstract:The AHNAK scaffold PDZ-protein family is implicated in various cellular processes including membrane repair; however, AHNAK function and subcellular localization in skeletal muscle are unclear. We used specific AHNAK1 and AHNAK2 antibodies to analyzed the detailed localization of both proteins in mouse skeletal muscle. Co-localization of AHNAK1 and AHNAK2 with vinculin clearly demonstrates that both proteins are components of the costameric network. In contrast, no AHNAK expression was detected in the T-tubule system. A laser wounding assay with AHNAK1-deficient fibers suggests that AHNAK1 is not involved in membrane repair. Using Atomic Force Microscopy (AFM), we observed a significantly higher transverse stiffness of AHNAK1-/- fibers. These findings suggest novel functions of AHNAK proteins in skeletal muscle.
Keywords:AHNAK1, AHNAK2, Costamer, Fiber Stiffness, Membrane Repair, Skeletal Muscle, Animals, Mice
Source:Biochemical and Biophysical Research Communications
ISSN:0006-291X
Publisher:Academic Press (U.S.A.)
Volume:401
Number:1
Page Range:143-148
Date:8 October 2010
Official Publication:https://doi.org/10.1016/j.bbrc.2010.09.030
PubMed:View item in PubMed

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