ZFP36L1 is regulated by growth factors and cytokines in keratinocytes and influences their VEGF production

Item Type:	Article
Title:	ZFP36L1 is regulated by growth factors and cytokines in keratinocytes and influences their VEGF production
Creators Name:	Hacker, C. and Valchanova, R. and Adams, S. and Munz, B.
Abstract:	Keratinocyte-derived growth factors and cytokines play an important role in epidermal homeostasis and particularly in cutaneous wound repair. Thus, we analyzed a potential role of the ZFP36/tristetraprolin family of zinc finger proteins, which are targets of these factors, but also regulate their production, in keratinocytes. We show that expression of ZFP36, ZFP36L1, and ZFP36L2 is induced by a broad variety of growth factors and cytokines, and by scratch wounding. Since ZFP36L1 is a modulator of vascular endothelium growth factor (VEGF) mRNA stability, we subsequently used siRNA technology to inhibit ZFP36L1 gene expression. Notably, this treatment resulted in prolonged maintenance of elevated VEGF levels in HaCaT keratinocytes upon epidermal growth factor stimulation of these cells. Taken together, our results suggest an important role of ZFP36L1 in wound healing.
Keywords:	Butyrate Response Factor 1, Cultured Cells, Cytokines, Gene Expression Profiling, Gene Expression Regulation, Intercellular Signaling Peptides and Proteins, Keratinocytes, Transcription Factors, Tristetraprolin, Vascular Endothelial Growth Factors, Wound Healing
Source:	Growth Factors
ISSN:	0897-7194
Publisher:	Taylor & Francis
Volume:	28
Number:	3
Page Range:	178-190
Date:	June 2010
Official Publication:	https://doi.org/10.3109/08977190903578660
PubMed:	View item in PubMed

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