Item Type: | Article |
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Title: | The landscape of C. elegans 3'UTRs |
Creators Name: | Mangone, M. and Manoharan, A.P. and Thierry-Mieg, D. and Thierry-Mieg, J. and Han, T. and Mackowiak, S. and Mis, E. and Zegar, C. and Gutwein, M.R. and Khivansara, V. and Attie, O. and Chen, K. and Salehi-Ashtiani, K. and Vidal, M. and Harkins, T.T. and Bouffard, P. and Suzuki, Y. and Sugano, S. and Kohara, Y. and Rajewsky, N. and Piano, F. and Gunsalus, K.C. and Kim, J.K. |
Abstract: | Three-prime untranslated regions (3'UTRs) of metazoan mRNAs contain numerous regulatory elements, yet remain largely uncharacterized. Using polyA capture, 3'RACE, full-length cDNAs, and RNA-seq, we define ~26,000 distinct 3'UTRs in Caenorhabditis elegans for ~85% of the 18,328 experimentally supported protein coding genes and revise ~40% of gene models. Alternative 3'UTR isoforms are frequent, often differentially expressed during development. Average 3'UTR length decreases with animal age. Surprisingly, no polyadenylation signal (PAS) is detected for 13% of polyA sites, predominantly among shorter alternative isoforms. Trans-spliced (vs. non-trans-spliced) mRNAs possess longer 3'UTRs and frequently contain no PAS or variant PAS. We identify conserved 3'UTR motifs, isoform-specific predicted microRNA target sites, and polyadenylation of most histone genes. Our data reveal a rich complexity of 3'UTRs genome-wide and throughout development. |
Keywords: | Binding Sites, Computational Biology, Conserved Sequence, Developmental Gene Expression Regulation, Gene Library, Helminth Genes, Helminth Proteins, Helminth RNA, Hermaphroditism, Histones, Messenger RNA, MicroRNA, Operon, Poly A, Polyadenylation, Trans-Splicing, Animals, Caenorhabditis elegans |
Source: | Science |
ISSN: | 0036-8075 |
Publisher: | American Association for the Advancement of Science |
Volume: | 329 |
Number: | 5990 |
Page Range: | 432-435 |
Date: | 23 July 2010 |
Official Publication: | https://doi.org/10.1126/science.1191244 |
PubMed: | View item in PubMed |
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