The landscape of C. elegans 3'UTRs

Item Type:	Article
Title:	The landscape of C. elegans 3'UTRs
Creators Name:	Mangone, M. and Manoharan, A.P. and Thierry-Mieg, D. and Thierry-Mieg, J. and Han, T. and Mackowiak, S. and Mis, E. and Zegar, C. and Gutwein, M.R. and Khivansara, V. and Attie, O. and Chen, K. and Salehi-Ashtiani, K. and Vidal, M. and Harkins, T.T. and Bouffard, P. and Suzuki, Y. and Sugano, S. and Kohara, Y. and Rajewsky, N. and Piano, F. and Gunsalus, K.C. and Kim, J.K.
Abstract:	Three-prime untranslated regions (3'UTRs) of metazoan mRNAs contain numerous regulatory elements, yet remain largely uncharacterized. Using polyA capture, 3'RACE, full-length cDNAs, and RNA-seq, we define ~26,000 distinct 3'UTRs in Caenorhabditis elegans for ~85% of the 18,328 experimentally supported protein coding genes and revise ~40% of gene models. Alternative 3'UTR isoforms are frequent, often differentially expressed during development. Average 3'UTR length decreases with animal age. Surprisingly, no polyadenylation signal (PAS) is detected for 13% of polyA sites, predominantly among shorter alternative isoforms. Trans-spliced (vs. non-trans-spliced) mRNAs possess longer 3'UTRs and frequently contain no PAS or variant PAS. We identify conserved 3'UTR motifs, isoform-specific predicted microRNA target sites, and polyadenylation of most histone genes. Our data reveal a rich complexity of 3'UTRs genome-wide and throughout development.
Keywords:	Binding Sites, Computational Biology, Conserved Sequence, Developmental Gene Expression Regulation, Gene Library, Helminth Genes, Helminth Proteins, Helminth RNA, Hermaphroditism, Histones, Messenger RNA, MicroRNA, Operon, Poly A, Polyadenylation, Trans-Splicing, Animals, Caenorhabditis elegans
Source:	Science
ISSN:	0036-8075
Publisher:	American Association for the Advancement of Science
Volume:	329
Number:	5990
Page Range:	432-435
Date:	23 July 2010
Official Publication:	https://doi.org/10.1126/science.1191244
PubMed:	View item in PubMed

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