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The SILAC fly allows for accurate protein quantification in vivo

Item Type:Article
Title:The SILAC fly allows for accurate protein quantification in vivo
Creators Name:Sury, M.D. and Chen, J.X. and Selbach, M.
Abstract:Stable isotope labeling by amino acids in cell culture (SILAC) is widely used to quantify protein abundance in tissue culture cells. Until now, the only multicellular organism completely labeled at the amino acid level is the laboratory mouse. The fruit fly Drosophila melanogaster is one of the most widely used small animal models in biology. Here, we show that feeding flies with SILAC labeled yeast leads to almost complete labeling in the first filial generation. We used these SILAC flies to investigate sexual dimorphism of protein abundance in D. melanogaster. Quantitative proteome comparison of adult male and female flies revealed distinct biological processes specific for each sex. Using a tudor mutant that is defective for germ cell generation allowed us to differentiate between sex-specific protein expression in the germline and somatic tissue. We identified many proteins with known sex-specific expression bias. In addition, several new proteins with a potential role in sexual dimorphism were identified. Collectively, our data shows that the SILAC fly can be used to accurately quantify protein abundance in vivo. The approach is simple, fast, and cost-effective which makes SILAC flies an attractive model system for the emerging field of in vivo quantitative proteomics.
Keywords:D. Melanogaster, Developmental Biology, Mass Spectrometry, Quantification, SILAC, Systems Biology, Animals, Drosophila Melanogaster
Source:Molecular & Cellular Proteomics
ISSN:1535-9476
Publisher:American Society for Biochemistry and Molecular Biology (U.S.A.)
Volume:9
Number:10
Page Range:2173-2183
Date:October 2010
Official Publication:https://doi.org/10.1074/mcp.M110.000323
PubMed:View item in PubMed

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