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Generating knockout rats by transposon mutagenesis in spermatogonial stem cells

Official URL:https://doi.org/10.1038/nmeth.1461
PubMed:View item in PubMed
Creators Name:Izsvak, Z. and Froehlich, J. and Grabundzija, I. and Shirley, J.R. and Powell, H.M. and Chapman, K.M. and Ivics, Z. and Hamra, F.K.
Journal Title:Nature Methods
Journal Abbreviation:Nat Methods
Volume:7
Number:6
Page Range:443-445
Date:June 2010
Keywords:DNA Transposable Elements, Gene Knockout Techniques, Mutagenesis, Spermatogonia, Stem Cells, Animals, Rats
Abstract:Disrupting genes in the rat on a genome-wide scale will allow the investigation of many biological processes linked to human health. Here we used transposon-mediated mutagenesis to knock out genes in rat spermatogonial stem cells. Given the capacity of the testis to support spermatogenesis from thousands of transplanted, genetically manipulated spermatogonia, this approach paves a way for high-throughput functional genomic studies in the laboratory rat.
ISSN:1548-7091
Publisher:Nature Publishing Group (U.S.A.)
Item Type:Article

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