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YB-1 relocates to the nucleus in adenovirus-infected cells and facilitates viral replication by inducing E2 gene expression through the E2 late promoter

Item Type:Article
Title:YB-1 relocates to the nucleus in adenovirus-infected cells and facilitates viral replication by inducing E2 gene expression through the E2 late promoter
Creators Name:Holm, P.S. and Bergmann, S. and Juerchott, K. and Lage, H. and Brand, K. and Ladhoff, A. and Mantwill, K. and Curiel, D.T. and Dobbelstein, M. and Dietel, M. and Gaensbacher, B. and Royer, H.D.
Abstract:The adenovirus early proteins E1A and E1B-55kDa are key regulators of viral DNA replication, and it was thought that targeting of p53 by E1B-55kDa is essential for this process. Here we have identified a previously unrecognized function of E1B for adenovirus replication. We found that E1B-55kDa is involved in targeting the transcription factor YB-1 to the nuclei of adenovirus type 5-infected cells where it is associated with viral inclusion bodies believed to be sites of viral transcription and replication. We show that YB-1 facilitates E2 gene expression through the E2 late promoter thus controlling E2 gene activity at later stages of infection. The role of YB-1 for adenovirus replication was demonstrated with an E1-minus adenovirus vector containing a YB-1 transgene. In infected cells, AdYB-1 efficiently replicated and produced infectious progeny particles. Thus, adenovirus E1B-55kDa protein and the host cell factor YB-1 act jointly to facilitate adenovirus replication in the late phase of infection.
Keywords:Adenoviridae, Adenovirus E2 Proteins, Bacterial Antigens, Bacterial Proteins, CCAAT-Enhancer-Binding Proteins, Cell Line, Cell Nucleus, Cell Nucleus Active Transport, Complementary DNA, Confocal Microscopy, DNA-Binding Proteins, Electron Microscopy, Fluorescence Microscopy, Gene Expression Regulation, Hela Cells, NFI Transcription Factors, Northern Blotting, Promoter Regions, Protein Binding, Southern Blotting, Transcription Factors, Transgenes, Virus Replication, Western Blotting, Y-Box-Binding Protein 1
Source:Journal of Biological Chemistry
ISSN:0021-9258
Publisher:American Society for Biochemistry and Molecular Biology (U.S.A.)
Volume:277
Number:12
Page Range:10427-10434
Date:1 January 2002
Official Publication:http://www.jbc.org/cgi/content/abstract/M106955200v1
PubMed:View item in PubMed

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