Endosomal chloride-proton exchange rather than chloride conductance is crucial for renal endocytosis

Item Type:	Article
Title:	Endosomal chloride-proton exchange rather than chloride conductance is crucial for renal endocytosis
Creators Name:	Novarino, G. and Weinert, S. and Rickheit, G. and Jentsch, T.J.
Abstract:	Loss of the endosomal anion transport protein ClC-5 impairs renal endocytosis and underlies human Dent's disease. ClC-5 is thought to promote endocytosis by facilitating endosomal acidification through neutralization of proton pump currents. However, ClC-5 is a 2Cl(-)/H(+)-exchanger rather than a Cl(-) channel. We generated mice carrying the uncoupling E211A (unc) mutation that converts ClC-5 into a pure Cl(-) conductor. ATP-dependent acidification of renal endosomes was reduced in ClC-5 knockout mice, but normal in Clcn5(unc) mice. Surprisingly, however, their proximal tubular endocytosis was also impaired. Thus endosomal chloride concentration, which is raised by ClC-5 in exchange for protons accumulated by the H(+)-ATPase, may play a role in endocytosis.
Keywords:	Adenosine Triphosphate, Chloride Channels, Chlorides, Electrophysiological Phenomena, Endocytosis, Endosomes, Hydrogen-Ion Concentration, Kidney Diseases, Proximal Kidney Tubules, Mutant Proteins, Proteinuria, Proton-Translocating ATPases, Protons, Animals, Mice
Source:	Science
ISSN:	0036-8075
Publisher:	American Association for the Advancement of Science
Volume:	328
Number:	5984
Page Range:	1398-1401
Date:	11 June 2010
Official Publication:	https://doi.org/10.1126/science.1188070
PubMed:	View item in PubMed

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