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Endogenous purinergic signaling is required for osmotic volume regulation of retinal glial cells

Item Type:Article
Title:Endogenous purinergic signaling is required for osmotic volume regulation of retinal glial cells
Creators Name:Wurm, A. and Lipp, S. and Pannicke, T. and Linnertz, R. and Kruegel, U. and Schulz, A. and Faerber, K. and Zahn, D. and Grosse, J. and Wiedemann, P. and Chen, J. and Schoeneberg, T. and Illes, P. and Reichenbach, A. and Bringmann, A.
Abstract:Intense neuronal activity in the sensory retina is associated with a volume increase of neuronal cells (Uckermann et al., J. Neurosci. 2004, 24:10149) and a decrease in the osmolarity of the extracellular space fluid (Dmitriev et al., Vis. Neurosci. 1999, 16:1157). Here, we show the existence of an endogenous purinergic mechanism that prevents hypoosmotic swelling of retinal glial (Müller) cells in mice. In contrast to the cells from wild-type mice, hypoosmotic stress induced rapid swelling of glial cell somata in retinal slices from mice deficient in P2Y(1), adenosine A(1) receptors, or ecto-5'-nucleotidase (CD73). Consistently, glial cell bodies in retinal slices from wild-type mice displayed osmotic swelling when P2Y(1) or A(1) receptors, or CD73, were pharmacologically blocked. Exogenous ATP, UTP, and UDP inhibited glial swelling in retinal slices, while the swelling of isolated glial cells was prevented by ATP but not by UTP or UDP, suggesting that uracil nucleotides indirectly regulate the glial cell volume via activation of neuronal P2Y(4/6) and neuron-to-glia signaling. It is suggested that autocrine/paracrine activation of purinergic receptors and enzymes is crucially involved in the regulation of the glial cell volume.
Keywords:A(1) Receptor, CD73, Glia, Osmotic Swelling, P2Y(1), Retina, Animals, Mice
Source:Journal of Neurochemistry
ISSN:0022-3042
Publisher:Wiley-Blackwell (U.K.)
Volume:112
Number:5
Page Range:1261-1272
Date:March 2010
Official Publication:https://doi.org/10.1111/j.1471-4159.2009.06541.x
PubMed:View item in PubMed

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