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Comparative analysis of an experimental subcellular protein localization assay and in silico prediction methods

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Item Type:Article
Title:Comparative analysis of an experimental subcellular protein localization assay and in silico prediction methods
Creators Name:Hu, Y. and Lehrach, H. and Janitz, M.
Abstract:The subcellular localization of a protein can provide important information about its function within the cell. As eukaryotic cells and particularly mammalian cells are characterized by a high degree of compartmentalization, most protein activities can be assigned to particular cellular compartments. The categorization of proteins by their subcellular localization is therefore one of the essential goals of the functional annotation of the human genome. We previously performed a subcellular localization screen of 52 proteins encoded on human chromosome 21. In the current study, we compared the experimental localization data to the in silico results generated by nine leading software packages with different prediction resolutions. The comparison revealed striking differences between the programs in the accuracy of their subcellular protein localization predictions. Our results strongly suggest that the recently developed predictors utilizing multiple prediction methods tend to provide significantly better performance over purely sequence-based or homology-based predictions.
Keywords:Protein Localization, In Silico Predictions, Human Chromosome 21, Immunocytochemistry
Source:Journal of Molecular Histology
ISSN:1567-2379
Publisher:Springer (The Netherlands)
Volume:40
Number:5-6
Page Range:343-352
Date:October 2009
Official Publication:https://doi.org/10.1007/s10735-009-9247-9
PubMed:View item in PubMed

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