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Global transcriptomic analysis of murine embryonic stem cell-derived brachyury+ (T) cells

Item Type:Article
Title:Global transcriptomic analysis of murine embryonic stem cell-derived brachyury+ (T) cells
Creators Name:Doss, M.X. and Wagh, V. and Schulz, H. and Kull, M. and Kolde, R. and Pfannkuche, K. and Nolden, T. and Himmelbauer, H. and Vilo, J. and Hescheler, J. and Sachinidis, A.
Abstract:Brachyury+ mesodermal cell population with purity over 79% was obtained from differentiating brachyury embryonic stem cells (ESC) generated with brachyury promoter driven enhanced green fluorescent protein and puromycin-N-acetyltransferase. A comprehensive transcriptomic analysis of brachyury+ cells enriched with puromycin application from 6-day-old embryoid bodies (EBs), 6-day-old control EBs and undifferentiated ESCs led to identification of 1573 uniquely up-regulated and 1549 uniquely down-regulated transcripts in brachyury+ cells. Furthermore, transcripts up-regulated in brachyury+ cells have overrepresented the Gene Ontology annotations (cell differentiation, blood vessel morphogenesis, striated muscle development, placenta development and cell motility) and Kyoto Encyclopedia of Genes and Genomes pathway annotations (mitogen-activated protein kinase signaling and transforming growth factor beta signaling). Transcripts representing Larp2 and Ankrd34b are notably up-regulated in brachyury+ cells. Knockdown of Larp2 resulted in a significantly down-regulation BMP-2 expression, and knockdown of Ankrd34b resulted in alteration of NF-H, PPAR gamma and PECAM1 expression. The elucidation of transcriptomic signatures of ESCs-derived brachyury+ cells will contribute toward defining the genetic and cellular identities of presumptive mesodermal cells. Furthermore, there is a possible involvement of Larp2 in the regulation of the late mesodermal marker BMP-2. Ankrd34b might be a positive regulator of neurogenesis and a negative regulator of adipogenesis.
Keywords:Activated Receptor alpha, Mesoderm Formation, In Vitro, Gene, Expression, Protein, Differentiation, Commitment, Database, Animals, Xenopus
Source:Genes to Cells
Page Range:209-228
Date:March 2010
Official Publication:https://doi.org/10.1111/j.1365-2443.2010.01390.x

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