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Quantitative proteomics reveals subset-specific viral recognition in dendritic cells

Official URL:https://doi.org/10.1016/j.immuni.2010.01.013
PubMed:View item in PubMed
Creators Name:Luber, C.A. and Cox, J. and Lauterbach, H. and Fancke, B. and Selbach, M. and Tschopp, J. and Akira, S. and Wiegand, M. and Hochrein, H. and O'Keeffe, M. and Mann, M.
Journal Title:Immunity
Journal Abbreviation:Immunity
Volume:32
Number:2
Page Range:279-289
Date:26 February 2010
Keywords:Signal Transducing Adaptor Proteins, CD Antigens, Cell Separation, Cultured Cells, DEAD-box RNA Helicases, Dendritic Cells, Flow Cytometry, Host-Pathogen Interactions, Mass Spectrometry, Myeloid Differentiation Factor 88, Proteomics, Respirovirus Infections, Sendai Virus, Animals, Mice
Abstract:Dendritic cell (DC) populations consist of multiple subsets that are essential orchestrators of the immune system. Technological limitations have so far prevented systems-wide accurate proteome comparison of rare cell populations in vivo. Here, we used high-resolution mass spectrometry-based proteomics, combined with label-free quantitation algorithms, to determine the proteome of mouse splenic conventional and plasmacytoid DC subsets to a depth of 5,780 and 6,664 proteins, respectively. We found mutually exclusive expression of pattern recognition pathways not previously known to be different among conventional DC subsets. Our experiments assigned key viral recognition functions to be exclusively expressed in CD4(+) and double-negative DCs. The CD8alpha(+) DCs largely lack the receptors required to sense certain viruses in the cytoplasm. By avoiding activation via cytoplasmic receptors, including retinoic acid-inducible gene I, CD8alpha(+) DCs likely gain a window of opportunity to process and present viral antigens before activation-induced shutdown of antigen presentation pathways occurs.
ISSN:1074-7613
Publisher:Cell Press (U.S.A.)
Item Type:Article

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