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Completely functional double-barreled chloride channel expressed from a single Torpedo cDNA

Item Type:Article
Title:Completely functional double-barreled chloride channel expressed from a single Torpedo cDNA
Creators Name:Bauer, C.K. and Steinmeyer, K. and Schwarz, J.R. and Jentsch, T.J.
Abstract:We have performed an electrophysiological analysis of the recently cloned Torpedo marmorata Cl- channel. Functional expression of Cl- channels in oocytes of Xenopus laevis previously injected with cRNA yielded an outward-rectifying current activated by hyperpolarization. Replacement of Cl- with other anions significantly reduced or inhibited the current. Single-channel recordings from cell-attached patches exhibited burst-like Cl- channel activity with rapid fluctuations between three equally spaced substates (0 pS, 9 pS, and 18 pS). The properties of the cloned Cl- channel were almost identical to those of the reconstituted native T. californica Cl- channel and were in full agreement with the predictions of the double-barreled channel model [Hanke, W. & Miller, C. (1983) J. Gen. Physiol. 82, 25-45]. Our results imply that the cloned cDNA codes for the completely functional Torpedo electroplax Cl- channel.
Keywords:Chloride Channels, Chlorides, DNA, Electric Conductivity, Electrophysiology, Ion Channel Gating, Ion Channels, Membrane Potentials, Membrane Proteins, Biological Models, Oocytes, Probability, Torpedo, Animals, Xenopus Laevis
Source:Proceedings of the National Academy of Sciences of the United States of America
ISSN:0027-8424
Publisher:National Academy of Sciences (U.S.A.)
Volume:88
Number:24
Page Range:11052-11056
Date:15 December 1991
Official Publication:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC53071/
PubMed:View item in PubMed

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