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Low single channel conductance of the major skeletal muscle chloride channel, ClC-1

Item Type:Article
Title:Low single channel conductance of the major skeletal muscle chloride channel, ClC-1
Creators Name:Pusch, M. and Steinmeyer, K. and Jentsch, T.J.
Abstract:We expressed the skeletal muscle chloride channel, ClC-1, in HEK293 cells and investigated it with the patch-clamp technique. Macroscopic properties are similar to those obtained after expression in Xenopus oocytes, except that faster gating kinetics are observed in mammalian cells. Nonstationary noise analysis revealed that both rat and human ClC-1 have a low single channel conductance of about 1 pS. This finding may explain the lack of single-channel data for chloride channels from skeletal muscle despite its high macroscopic chloride conductance.
Keywords:Cell Line, Chloride Channels, Electrophysiology, Mammalian Embryo, Nonmammalian Embryo, Kidneys, Membrane Potentials, Muscle Proteins, Muscles, Oocytes, Transfection, Animals, Rats, Xenopus
Source:Biophysical Journal
ISSN:0006-3495
Publisher:Biophysical Society (U.S.A.)
Volume:66
Number:1
Page Range:149-152
Date:January 1994
Official Publication:https://doi.org/10.1016/S0006-3495(94)80753-2
PubMed:View item in PubMed

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