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Cloning and functional expression of rat CLC-5, a chloride channel related to kidney disease

Item Type:Article
Title:Cloning and functional expression of rat CLC-5, a chloride channel related to kidney disease
Creators Name:Steinmeyer, K. and Schwappach, B. and Bens, M. and Vandewalle, A. and Jentsch, T.J.
Abstract:We have cloned a novel member of the CLC chloride channel family from rat brain, rCLC-5. The cDNA predicts a 83-kDa protein belonging to the branch including CLC-3 and CLC-4, with which it shares approximately 80% identity. Expression of rCLC-5 in Xenopus oocytes elicits novel anion currents. They are strongly outwardly rectifying and have a conductivity sequence of NO3- > Cl- > Br- > I- > glutamate-. Although CLC-5 has consensus sites for phosphorylation by protein kinase A, raising the intracellular cAMP concentration had no effect on these currents. Currents were also unchanged when rCLC-5 was coexpressed with rCLC-3 and rCLC-4, either singly or in combination. rCLC-5 is expressed predominantly in kidney and also in brain, lung, and liver. Along the nephron, rCLC-5 message is detectable in all tubule segments investigated, but expression in the glomerulus and the S2 segment of the proximal tubule is low.
Keywords:Base Sequence, Chloride Channels, Molecular Cloning, Cyclic AMP, Cyclic AMP-Dependent Protein Kinases, DNA Primers, Complementary DNA, Kidney Diseases, Molecular Sequence Data, Phosphorylation, Amino Acid Sequence Homology, Animals, Rats, Xenopus
Source:Journal of Biological Chemistry
Publisher:American Society for Biochemistry and Molecular Biology
Page Range:31172-31177
Date:29 December 1995
Official Publication:https://doi.org/10.1074/jbc.270.52.31172
PubMed:View item in PubMed

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