Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

Membrane properties of ameboid microglial cells in the corpus callosum slice from early postnatal mice

[img] PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
2MB

Item Type:Article
Title:Membrane properties of ameboid microglial cells in the corpus callosum slice from early postnatal mice
Creators Name:Brockhaus, J. and Ilschner, S. and Banati, R.B. and Kettenmann, H.
Abstract:Microglial cells in culture are distinct from neurons, macroglial cells, and macrophages of tissues other than brain with respect to their membrane current pattern. To assess these cells in the intact tissue, we have applied the patch-clamp technique to study membrane currents in microglial cells from acute, whole brain slices of 6-9-d-old mice in an area of microglial cell invasion, the cingulum. As strategies to identify microglial cells prior to or after recording, we used binding and incorporation of Dil-acetylated low-density lipoproteins, binding of fluorescein isothiocyanate-coupled IgG via microglial Fc-receptors, and ultrastructural characterization. As observed previously for cultured microglial cells, depolarizing voltage steps activate only minute if any membrane currents, while hyperpolarizing voltage steps induced large inward currents. These currents exhibited properties of the inwardly rectifying K+ channel in that the reversal potential depended on the transmembrane K+ gradient, inactivation time constants decreased with hyperpolarization, and the current was blocked by tetraethylammonium (50 mM). This study represents the first attempt to assess microglial cells in situ using electrophysiological methods. It opens the possibility to address questions related to the function of microglial cells in the intact CNS.
Keywords:Microglia, Brain Macrophages, Electrophysiology, Patch-Clamp Technique, K+ Channels, Brain Slices, Cingulum, Development, Animals, Mice
Source:Journal of Neuroscience
ISSN:0270-6474
Publisher:Society for Neuroscience (U.S.A.)
Volume:13
Number:10
Page Range:4412-4421
Date:October 1993
Additional Information:Copyright (c) 1993 by The Society for Neuroscience
Official Publication:http://www.jneurosci.org/cgi/content/abstract/13/10/4412
PubMed:View item in PubMed

Repository Staff Only: item control page

Downloads

Downloads per month over past year

Open Access
MDC Library