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CLC chloride channels in Caenorhabditis elegans

Item Type:Article
Title:CLC chloride channels in Caenorhabditis elegans
Creators Name:Schriever, A.M. and Friedrich, T. and Pusch, M. and Jentsch, T.J.
Abstract:The genome of the nematode Caenorhabditis elegans encodes six putative chloride channels (CeCLC-1 through CeCLC-6) that represent all three known branches of the mammalian CLC gene family. Using promoter fragments to drive the expression of the green fluorescent protein, CeCLC-2, -3, and -4 expression was studied in transgenic C. elegans. CeCLC-4 was specifically expressed in the large H-shaped excretory cell, where it was co-expressed with CeCLC-3, which is also expressed in other cells, including neurons, muscles, and epithelial cells. Also, CeCLC-2 was expressed in several cells of the nervous system, intestinal cells, and vulval muscle cells. Similar to mammalian CLC proteins, only two nematode CLC channels elicited detectable plasma membrane currents in Xenopus oocytes. CeCLC-3 currents were inwardly rectifying and were activated by positive prepulses. Its complex gating behavior can be explained by two gates, at least one of which depends on extracellular anions. In this respect it resembles some mammalian chloride channels with which it also shares a preference of chloride over iodide. C. elegans thus provides new opportunities to understand common mechanisms underlying structure and function in CLC channels and will allow for a genetic dissection of chloride channels in this simple model organism.
Keywords:Cell Line, Chloride Channels, Chlorides, Complementary DNA, Electrophysiology, Gene Expression, Helminth Genes, Green Fluorescent Proteins, Hydrogen-Ion Concentration, Luminescent Proteins, Membrane Potentials, Molecular Sequence Data, Oocytes, Phylogeny, Protein Isoforms, RNA Splicing, Recombinant Fusion Proteins, DNA Sequence Analysis, Animals, Caenorhabditis Elegans, Xenopus
Source:Journal of Biological Chemistry
Publisher:American Society for Biochemistry and Molecular Biology
Page Range:34238-34244
Date:26 November 1999
Official Publication:https://doi.org/10.1074/jbc.274.48.34238
PubMed:View item in PubMed

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