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Inhibition of KCNQ1-4 potassium channels expressed in mammalian cells via M1 muscarinic acetylcholine receptors

Item Type:Article
Title:Inhibition of KCNQ1-4 potassium channels expressed in mammalian cells via M1 muscarinic acetylcholine receptors
Creators Name:Selyanko, A.A. and Hadley, J.K. and Wood, I.C. and Abogadie, F.C. and Jentsch, T.J. and Brown, D.A.
Abstract:1. KCNQ1-4 potassium channels were expressed in mammalian Chinese hamster ovary (CHO) cells stably transfected with M1 muscarinic acetylcholine receptors and currents were recorded using the whole-cell perforated patch technique and cell-attached patch recording. 2. Stimulation of M1 receptors by 10 microM oxotremorine-M (Oxo-M) strongly reduced (to 0-10%) currents produced by KCNQ1-4 subunits expressed individually and also those produced by KCNQ2 + KCNQ3 and KCNQ1 + KCNE1 heteromers, which are thought to generate neuronal M-currents (IK,M) and cardiac slow delayed rectifier currents (IK,s), respectively. 3. The activity of KCNQ2 + KCNQ3, KCNQ2 and KCNQ3 channels recorded with cell-attached pipettes was strongly and reversibly reduced by Oxo-M applied to the extra-patch membrane. 4. It is concluded that M1 receptors couple to all known KCNQ subunits and that inhibition of KCNQ2 + KCNQ3 channels, like that of native M-channels, requires a diffusible second messenger.
Keywords:CHO Cells, KCNQ Potassium Channels, Muscarinic Agonists, Oxotremorine, Pat-Clamp Techniques, Potassium Channel Blockersch, Voltage-Gated Potassium Channels, Muscarinic M1 Receptor, Muscarinic Receptors, Chemical Stimulation, Transfection, Animals, Cricetinae
Source:Journal of Physiology
ISSN:0022-3751
Publisher:Cambridge University Press (U.K.)
Volume:522
Number:Pt 3
Page Range:349-355
Date:1 February 2000
Official Publication:https://doi.org/10.1111/j.1469-7793.2000.t01-2-00349.x
PubMed:View item in PubMed

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