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Disruption of ClC-3, a chloride channel expressed on synaptic vesicles, leads to a loss of the hippocampus

Item Type:Article
Title:Disruption of ClC-3, a chloride channel expressed on synaptic vesicles, leads to a loss of the hippocampus
Creators Name:Stobrawa, S.M. and Breiderhoff, T. and Takamori, S. and Engel, D. and Schweizer, M. and Zdebik, A.A. and Boesl, M.R. and Ruether, K. and Jahn, H. and Draguhn, A. and Jahn, R. and Jentsch, T.J.
Abstract:Several plasma membrane chloride channels are well characterized, but much less is known about the molecular identity and function of intracellular Cl- channels. ClC-3 is thought to mediate swelling-activated plasma membrane currents, but we now show that this broadly expressed chloride channel is present in endosomal compartments and synaptic vesicles of neurons. While swelling-activated currents are unchanged in mice with disrupted ClC-3, acidification of synaptic vesicles is impaired and there is severe postnatal degeneration of the retina and the hippocampus. Electrophysiological analysis of juvenile hippocampal slices revealed no major functional abnormalities despite slightly increased amplitudes of miniature excitatory postsynaptic currents. Mice almost lacking the hippocampus survive and show several behavioral abnormalities but are still able to acquire motor skills.
Keywords:Acids, Animal Behavior, Chloride Channels, Chlorides, Electroretinography, Excitatory Postsynaptic Potentials, Gene Targeting, Growth Disorders, Hippocampus, Motor Activity, Pyramidal Cells, Retinal Degeneration, Synaptic Vesicles, Animals, Mice
Source:Neuron
ISSN:0896-6273
Publisher:Cell Press (U.S.A.)
Volume:29
Number:1
Page Range:185-196
Date:January 2001
Official Publication:https://doi.org/10.1016/S0896-6273(01)00189-1
PubMed:View item in PubMed

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