In HCMV-exposed HUVEC, P52/RelB regulatory factors mediate activation of the human PAR1 gene promoter

Item Type:	Article
Title:	In HCMV-exposed HUVEC, P52/RelB regulatory factors mediate activation of the human PAR1 gene promoter
Creators Name:	Popovic, M. and Paskas, S. and Zivkovic, M. and Duric-Delic, T. and Stankovic, A. and Burysek, L.
Abstract:	We studied the effect of HCMV (AD-169) on the regulation of PAR1 reporter activity in HUVEC. In HUVEC, HCMV significantly induced activation of PAR1 reporter constructs carrying AP-2-like and two complexes of AP-2/Sp1-binding elements. Shorter constructs with no AP-2-like binding elements had lower activity upon virus inoculation. Moreover, activity of the shortest construct without binding elements was not significantly increased in virus-exposed cells. Furthermore, in HCMV-exposed cells, activity of the PARI reporter was significantly augmented upon p52/RelB transfection. Taken together, the data indicate that activity of the PAR1 promoter is highly dependent on AP-2/Sp1 binding elements and mediated by p52/RelB regulatory factors.
Keywords:	HCMV, PAR1 Gene Reporter, HUVEC, Thrombin Receptor PAR-1, Endothelial Cells, Human Melanoma, Trafficking, Expression
Source:	Archives of Biological Sciences
ISSN:	0354-4664
Publisher:	Serbian Biological Society
Volume:	61
Number:	4
Page Range:	613-618
Date:	2009
Official Publication:	https://doi.org/10.2298/ABS0904613P

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