Temporal and tissue-specific expression of the MET ORF driven by the complete transcriptional unit of human A1AT gene in transgenic mice

Item Type:	Comment
Title:	Temporal and tissue-specific expression of the MET ORF driven by the complete transcriptional unit of human A1AT gene in transgenic mice
Creators Name:	Amicone, L. and Galimi, M.A. and Spagnoli, F.M. and Tommasini, C. and De Luca, V. and Tripodi, M.
Abstract:	We inserted the sequence coding for the cytoplasmic portion of the human MET receptor into an 18-kb genomic fragment containing the entire human A1AT gene (encoding alpha-1-antitrypsin). Stringent control of gene expression, at the transcriptional, post-transcriptional and translational levels, was ensured by insertion of the MET open reading frame into A1AT, thus maintaining: (i) all the elements that confer tissue-specific transcription initiation, (ii) all the sequences involved in transcript processing and (iii) all the sequences which influence messenger stability and translational efficiency. The expression pattern of this vector in transgenic mice was identical to that of the human A1AT transgene, as well as to that of A1AT in humans with regard to both temporal and tissue-specific regulation.
Keywords:	Gene Expression Control, alpha-l-Antitrypsin, Homologous Recombination Procedure of Cloning, Site-Directed Mutagenesis, Expression Vector, Animals, Mice
Source:	Gene
ISSN:	0378-1119
Publisher:	Elsevier
Volume:	162
Number:	2
Page Range:	323-328
Date:	11 September 1995
Official Publication:	https://doi.org/10.1016/0378-1119(95)00277-D
PubMed:	View item in PubMed

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