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Evidence for a protein tether involved in somatic touch

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Official URL:https://doi.org/10.1038/emboj.2009.398
PubMed:View item in PubMed
Creators Name:Hu, J. and Chiang, L.Y. and Koch, M. and Lewin, G.R.
Journal Title:EMBO Journal
Journal Abbreviation:EMBO J
Volume:29
Number:4
Page Range:855-867
Date:17 February 2010
Keywords:Extracellular Matrix, Ion Channels, Laminin, Mechanotransduction, Proteases, Animals, Mice
Abstract:The gating of ion channels by mechanical force underlies the sense of touch and pain. The mode of gating of mechanosensitive ion channels in vertebrate touch receptors is unknown. Here we show that the presence of a protein link is necessary for the gating of mechanosensitive currents in all low-threshold mechanoreceptors and some nociceptors of the dorsal root ganglia (DRG). Using TEM, we demonstrate that a protein filament with of length approximately 100 nm is synthesized by sensory neurons and may link mechanosensitive ion channels in sensory neurons to the extracellular matrix. Brief treatment of sensory neurons with non-specific and site-specific endopeptidases destroys the protein tether and abolishes mechanosensitive currents in sensory neurons without affecting electrical excitability. Protease-sensitive tethers are also required for touch-receptor function in vivo. Thus, unlike the majority of nociceptors, cutaneous mechanoreceptors require a distinct protein tether to transduce mechanical stimuli.
ISSN:0261-4189
Publisher:Nature Publishing Group (U.S.A.)
Item Type:Article

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