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Targets of the Tal1 transcription factor in erythrocytes: E2 ubiquitin conjugase regulation by Tal1

Item Type:Article
Title:Targets of the Tal1 transcription factor in erythrocytes: E2 ubiquitin conjugase regulation by Tal1
Creators Name:Lausen, J. and Pless, O. and Leonard, F. and Kuvardina-Bolling, O.N. and Koch, B. and Leutz, A.
Abstract:The Tal1 transcription factor is essential for the development of the hematopoietic system and plays a role during definitive erythropoiesis in the adult. Despite the importance of Tal1 in erythropoiesis only a small number of erythroid differentiation target genes are known. A chromatin precipitation and cloning approach was established to uncover novel Tal1 target genes in erythropoiesis. The BirA-tag/BirA-ligase biotinylation system in combination with streptavidine chromatin precipitation (Strep-CP) was used to co-precipitate genomic DNA bound to Tal1. Tal1 was found to bind in the vicinity of 31 genes including the E2-ubiquitin conjugase UBE2H gene. Binding of Tal1 to UBE2H was confirmed by chromatin immuno-precipitation. UBE2H expression is increased during erythroid differentiation of hCD34+ cells. Tal1 expression activated UBE2H expression whereas Tal1 knock-down reduced UBE2H expression and ubiquitin transfer activity. This study identifies parts of the ubiquitinylation machinery as a cellular target down-stream of the transcription factor Tal1 and provides novel insights into Tal1 regulated erythropoiesis.
Keywords:Blood Erythropoeisis, Chromatin/Immunoprecipitation/ChIP, Development Differentiation/Hematopoietic, Diseases/Cancer/Leukemia, Gene/Transcription, Proteases/Ubiquitination, Transcription
Source:Journal of Biological Chemistry
ISSN:0021-9258
Publisher:American Society for Biochemistry and Molecular Biology (U.S.A.)
Volume:285
Number:8
Page Range:5338-5346
Date:19 February 2010
Official Publication:https://doi.org/10.1074/jbc.M109.030296
PubMed:View item in PubMed

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