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Proteome organization in a genome-reduced bacterium

Item Type:Article
Title:Proteome organization in a genome-reduced bacterium
Creators Name:Kuehner, S. and van Noort, V. and Betts, M.J. and Leo-Macias, A. and Batisse, C. and Rode, M. and Yamada, T. and Maier, T. and Bader, S. and Beltran-Alvarez, P. and Castano-Diez, D. and Chen, W.H. and Devos, D. and Gueell, M. and Norambuena, T. and Racke, I. and Rybin, V. and Schmidt, A. and Yus, E. and Aebersold, R. and Herrmann, R. and Boettcher, B. and Frangakis, A.S. and Russell, R.B. and Serrano, L. and Bork, P. and Gavin, A.C.
Abstract:The genome of Mycoplasma pneumoniae is among the smallest found in self-replicating organisms. To study the basic principles of bacterial proteome organization, we used tandem affinity purification-mass spectrometry (TAP-MS) in a proteome-wide screen. The analysis revealed 62 homomultimeric and 116 heteromultimeric soluble protein complexes, of which the majority are novel. About a third of the heteromultimeric complexes show higher levels of proteome organization, including assembly into larger, multiprotein complex entities, suggesting sequential steps in biological processes, and extensive sharing of components, implying protein multifunctionality. Incorporation of structural models for 484 proteins, single-particle electron microscopy, and cellular electron tomograms provided supporting structural details for this proteome organization. The data set provides a blueprint of the minimal cellular machinery required for life.
Keywords:Bacterial Proteins, Computational Biology, Bacterial Genome, Mass Spectrometry, Metabolic Networks and Pathways, Electron Microscopy, Biological Models, Molecular Models, Multiprotein Complexes, Mycoplasma pneumoniae, Automated Pattern Recognition, Protein Interaction Mapping, Proteome, Systems Biology
Source:Science
ISSN:0036-8075
Publisher:American Association for the Advancement of Science (U.S.A.)
Volume:326
Number:5957
Page Range:1235-1240
Date:27 November 2009
Official Publication:https://doi.org/10.1126/science.1176343
PubMed:View item in PubMed

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