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Transcriptome complexity in a genome-reduced bacterium

Item Type:Article
Title:Transcriptome complexity in a genome-reduced bacterium
Creators Name:Gueell, M. and van Noort, V. and Yus, E. and Chen, W.H. and Leigh-Bell, J. and Michalodimitrakis, K. and Yamada, T. and Arumugam, M. and Doerks, T. and Kuehner, S. and Rode, M. and Suyama, M. and Schmidt, S. and Gavin, A.C. and Bork, P. and Serrano, L.
Abstract:To study basic principles of transcriptome organization in bacteria, we analyzed one of the smallest self-replicating organisms, Mycoplasma pneumoniae. We combined strand-specific tiling arrays, complemented by transcriptome sequencing, with more than 252 spotted arrays. We detected 117 previously undescribed, mostly noncoding transcripts, 89 of them in antisense configuration to known genes. We identified 341 operons, of which 139 are polycistronic; almost half of the latter show decaying expression in a staircase-like manner. Under various conditions, operons could be divided into 447 smaller transcriptional units, resulting in many alternative transcripts. Frequent antisense transcripts, alternative transcripts, and multiple regulators per gene imply a highly dynamic transcriptome, more similar to that of eukaryotes than previously thought.
Keywords:Base Sequence, Gene Expression Profiling, Bacterial Gene Expression Regulation, Bacterial Genes, Molecular Sequence Data, Mycoplasma pneumoniae, Oligonucleotide Array Sequence Analysis, Operon, Antisense RNA, Bacterial RNA, Messenger RNA, Untranslated RNA, Genetic Transcription
Source:Science
ISSN:0036-8075
Publisher:American Association for the Advancement of Science (U.S.A.)
Volume:326
Number:5957
Page Range:1268-1271
Date:27 November 2009
Official Publication:https://doi.org/10.1126/science.1176951
PubMed:View item in PubMed

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