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Isolation of naturally associated bacteria of necromenic Pristionchus nematodes and fitness consequences

Item Type:Article
Title:Isolation of naturally associated bacteria of necromenic Pristionchus nematodes and fitness consequences
Creators Name:Rae, R. and Riebesell, M. and Dinkelacker, I. and Wang, Q. and Herrmann, M. and Weller, A.M. and Dieterich, C. and Sommer, R.J.
Abstract:Nematodes and bacteria are major components of the soil ecosystem. Many nematodes use bacteria for food, whereas others evolved specialized bacterial interactions ranging from mutualism to parasitism. Little is known about the biological mechanisms by which nematode-bacterial interactions are achieved, largely because in the laboratory nematodes are often cultured under artificial conditions. We investigated the bacterial interactions of nematodes from the genus Pristionchus that have a strong association with scarab beetles. Pristionchus has a different feeding strategy than Caenorhabditis and meta-genomic 16S sequence analysis of Pristionchus individuals showed a diversity of living bacteria within the nematode gut and on the nematode cuticle. Twenty-three different bacterial strains were isolated from three Pristionchus-beetle associations and were used to study nematode-bacterial interactions under controlled laboratory conditions. We show a continuum of bacterial interactions from dissemination, to reduction in brood size and nematode mortality caused by bacteria derived from insect hosts. Olfactory discrimination experiments show distinct chemoattraction and fitness profiles of Pristionchus nematodes when exposed to different bacteria. For example, Pristionchus pacificus avoids Serratia marcescens possibly because of pathogenicity. Also, P. pacificus avoids Bacillus thuringiensis and insect pathogenic bacteria but is resistant to the human pathogens Staphylococcus aureus and Pseudomonas aeruginosa, unlike Caenorhabditis elegans. Pristionchus specifically recognize and respond to bacteria that cause ill health. Bringing the nematode-bacterial interaction into the laboratory allows detailed functional studies, including the genetic manipulation of the interaction in both nematodes and bacteria.
Keywords:Pristionchus Pacificus, Caenorhabditis Elegans, Nematode-Bacterial Interactions, Bacillus Thuringiensis, Entomopathogenic Bacteria, Animals, Beetles
Source:Journal of Experimental Biology
ISSN:0022-0949
Publisher:Company of Biologists (U.K.)
Volume:211
Number:Pt 12
Page Range:1927-1936
Date:June 2008
Official Publication:https://doi.org/10.1242/jeb.014944
PubMed:View item in PubMed

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